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Core Workflow

ggpicrust2()
This function integrates pathway name/description annotations, ten of the most advanced differential abundance (DA) methods, and visualization of DA results.
ko2kegg_abundance()
Convert KO abundance in picrust2 export files to KEGG pathway abundance
pathway_daa()
Differential Abundance Analysis for Predicted Functional Pathways
pathway_annotation()
Pathway information annotation
pathway_errorbar()
The function pathway_errorbar() is used to visualize the results of functional pathway differential abundance analysis as error bar plots.
pathway_errorbar_table()
Generate Abundance Statistics Table for Pathway Analysis
pathway_heatmap()
Create pathway heatmap with support for multiple grouping variables
pathway_pca()
Perform Principal Component Analysis (PCA) on functional pathway abundance data
pathway_volcano()
Volcano Plot for Pathway Differential Abundance Analysis
pathway_ridgeplot()
Ridge Plot for GSEA Results
compare_daa_results()
Compare the Consistency of Statistically Significant Features
compare_metagenome_results()
Compare Metagenome Results

Taxa Contribution

read_contrib_file()
Read PICRUSt2 contribution file
read_pathway_contrib_file()
Read PICRUSt2 pathway-level contribution file
read_strat_file()
Read PICRUSt2 stratified abundance file
aggregate_taxa_contributions()
Aggregate taxa contributions for visualization
taxa_contribution_bar()
Stacked bar plot of taxa contributions
taxa_contribution_heatmap()
Heatmap of taxa contributions across functions

GSEA

pathway_gsea()
Gene Set Enrichment Analysis for PICRUSt2 output
visualize_gsea()
Visualize GSEA results
compare_gsea_daa()
Compare GSEA and DAA results
gsea_pathway_annotation()
Annotate GSEA results with pathway information
prepare_gene_sets()
Prepare gene sets for GSEA

Data

metadata
Metadata for ggpicrust2 Demonstration
ko_abundance
KO Abundance Dataset
kegg_abundance
KEGG Abundance Dataset
metacyc_abundance
MetaCyc Abundance Dataset
daa_results_df
DAA Results Dataset
daa_annotated_results_df
Differentially Abundant Analysis Results with Annotation

Reference Data

ko_reference
KEGG Orthology (KO) Reference Dataset
kegg_pathway_reference
KEGG Pathway Name Reference Dataset
ec_reference
EC Number Reference Dataset
metacyc_reference
MetaCyc Pathway Reference Dataset
ko_to_kegg_reference
KO to KEGG Pathway Reference Data
ko_to_go_reference
KO to GO Reference Mapping Dataset
metacyc_to_ec_reference
MetaCyc Pathway to EC Number Mapping Dataset

Utilities

import_MicrobiomeAnalyst_daa_results()
Import Differential Abundance Analysis (DAA) results from MicrobiomeAnalyst
color_themes
Color Theme System for ggpicrust2
legend_annotation_utils
Legend and Annotation Utilities for ggpicrust2
create_gradient_colors()
Create Gradient Colors
create_legend_theme()
Create Enhanced Legend Theme
create_pathway_class_theme()
Create Pathway Class Annotation Theme
get_available_themes()
Get Available Color Themes
get_color_theme()
Get Color Theme
get_significance_colors()
Get Significance Colors
get_significance_stars()
Get Significance Stars
calculate_smart_text_size()
Smart Text Size Calculator
preview_color_theme()
Preview Color Theme
format_pvalue_smart()
Smart P-value Formatting
smart_color_selection()
Smart Color Selection
resolve_annotation_overlaps()
Detect and Resolve Annotation Overlaps
safe_extract()
Safely Extract Elements from a List